Uji Aktivitas Antioksidan Ekstrak Daun Salam (Syzygium Polyanthum) Dengan Menggunanakan 1,1-Difenil-2-Pikrilhidrazil

Nurdin Rahman, Putrawan Bahriul, Anang Wahid M. Diah

Abstract


Antioxidant activity test of bay leave (Syzygium polyanthum) extract using 1,1-diphenyl-2-picrylhidrazyl (DPPH) has been investigated. The aim of this research is to determine antioxidant strength of bay leave (Syzygium polyanthum) extract. The bay leaves used in this research were young, medium, and old leaves categories. Concentration of DPPH free radical after additional bay leave extracts were determined by UV-Vis spectrophotometry. Variation concentrations of bay leave extracts were 20 ppm, 40 ppm, 60 ppm, and 80 ppm. Vitamin C solution was used as the positive control at the similar concentration, while DPPH solution in ethanol absolute was the negative control. The results showed that IC50 values for young, middle, and old leaves extracts were 37.441 ppm, 14.889 ppm, and 11.001 ppm, whereas vitamin C was 9.898 ppm. Based on these IC50 values, vitamin C is the strongest antioxidant than young, middle, and old bay leave extracts, and these extracts are classified as very strong natural antioxidant.

Keywords


Antioxidant Activity; Exstract Bay Leave; Bay Leave; DPPH; Vitamin C

References


Arianti, Harsojo, Syafria, Y., & Ermayanti, T. M. (2007). Isolasi dan uji antibakteri batang sambung nyawa (gynura procumbens lour) umur panen 1, 4 dan 7 bulan. Jurnal Bahan Alam Indonesia, 6(2), 43-45.

Ariviani, S. (2010). Anti radical capacity of anthosianin extract from fresh salam (syzygium polyanthum [Wight.] walp) fruits with varied solvent proportion. Caraka Tani, 25(1), 43-49.

Ghulamahdi, M., Aziz, S. A., & Nirwan. (2008). Peningkatan laju pertumbuhan dan kandungan flavonoid klon daun dewa (gynura pseudochina (L.) DC) melalui periode pencahayaan. Bul. Agronomi, 36(1), 40-48.

Harborne, J. B. (1987). Metode fitokimia: Penentuan cara modern menganalisis tumbuhan. Bandung: ITB.

Isnindar, Wahyuono, S., & Setyowati, E. P. (2011). Isolasi dan identifikasi senyawa antioksidan daun kesemek (diospyros kaki thunb.) dengan metode DPPH (2,2-Difenil-1 Pikrilhidrazil). Majalah Obat Tradisional, 16(3), 157-164.

Juniarti, Osmeli, D., & Yuhernita. (2009). Kandungan senyawa kimia, uji toksisitas (brine shrimp lethality test) dan antioksidan (1,1-diphenyl-2-pikrilhydrazyl) dari ekstrak daun saga (abrus precatorius L.). Makara Sains, 13(1), 50-54.

Khopkar, S. M. (2003). Konsep dasar kimia analitik. Jakarta: UI-Press.

Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., & Taniguchi, H. (2002). Antioxidants properties of ferulic acid and it’s related compound. Journal of Agricurtural Food Chemistry, 50(7), 2161-2168.

Kuntorini, E. M. (2013). Kemampuan antioksidan bulbus bawang dayak (eleutherine americana merr) pada umur berbeda. Diunduh kembali dari http:// jurnal.fmipa.unila.ac.id/index.php/ semirata/article/download/686/506.

Mailandari, M. (2012). Uji aktivitas antioksidan ekstrak daun garcinia kydia roxb. dengan metode DPPH dan identifikasi senyawa kimia fraksi yang aktif. Depok: Universitas Indonesia.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Journal of Science Technology, 26(2), 211-219.

Oke, J. M. & Hamburger, M. O. (2002). Screening of some nigerian medicinal plants for activity using 2,2-diphenyl-picryl-hidrazil (DPPH) radical. African Journal of Biomedical Research, 5(1), 77-79.

Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected iranian medicinal plants. African journal of Biotechnology, 5(11), 1142-1145.

Riansari, A. (2008). Pengaruh pemberian ekstrak daun salam (eugenia polyantha) terhadap kadar kolesterol totalserum tikus jantan galur wistar hiperlipidemia. Diunduh kembali dari http://eprints.undip. ac.id/24176/1/Anugerah_R.pdf.

Sembiring, S., Winarti, C., & Baringbing, B. (2008). Identifikasi komponen kimia minyak daun salam (eugenia polyantha) dari Sukabumi dan Bogor. Diunduh kembali dari http://balittro.litbang.deptan.go.id/ind/images/publikasi/bul.vol.14.no.2/Bagem-DaunSalam.pdf.

Sumono, A., & Mulan, A. (2009). Capability of boiling water of bay leaf (eugenia polyantha W.) for reducing streptococcus sp. colony. Majalah Farmasi Indonesia. 20(3), 112–117.

Talapessy, S., Suryanto, E., & Yudistira, A. (2013). Uji aktivitas antioksidan dari ampas hasil pengolahan sagu (metroxylon sagu rottb). Jurnal Ilmiah Farmasi, 2(3), 40-44.

Utomo, A. B., Suprijono, A., & Risdianto, A. (2008). Uji aktivitas antioksidan kombinasi ekstrak sarang semut (myrmecodia pendans) dan ekstrak teh hitam (camellia sinensis O.K.var.assamica (mast.)) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Diunduh kembali dari http://journal.stifar.ac.id/ojs/ index.php/js/article/viewFile/6/7.

Zuhra, C. F., Tarigan, J. B., & Sihotang, H. (2008). Aktivitas antioksidan senyawa flavonoid dari daun katuk (sauropus androgunus (L) Merr.). Jurnal Biologi Sumatera, 3(1), 7-10.


Full Text: PDF

Refbacks

  • There are currently no refbacks.



p-ISSN: 2302-6030; e-ISSN: 2477-5185

CODEN: JAKUA7


Creative Commons License

Jurnal Akademika Kimia
by Universitas Tadulako is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at
http://jurnal.untad.ac.id/jurnal/index.php/jak

Indexed By

Crossref WorldCat PKP Index OneSearch BASE Scholar GARUDA Sinta Neliti Mendeley