FORMULASI SUBSTRAT DASAR KOTORAN KAMBING DAN LIMBAH CAIR TEMPE DENGAN INOKULUM RUMEN SAPI UNTUK STUDI AWAL PRODUKSI BIOGAS

Abstract

Production of biogas using the basic substrate of tempeh’s liquid waste and goat’s waste with the bacteria inoculum of cow’s rumen has been experimentally conducted. This study aims to produce biogas using the substrate with the bacteria inoculum of cow’s rumen and know the optimal mixture ratio of the basic substrate and the inoculum. In this study, the amounts of basic substrate applied were 500 g chicken’s waste and 500 mL liquid waste of tempeh. The study was designed using Complete Random Design comprising 6 treatments with 3 times repetitions, respectively. The treatments tested were P₀ (100% basic substrate), P₁ (80% basic substrate + 20% inoculum), P₂ (60% basic substrate + 40% inoculum), P₃ (40% basic substrate + 60% inoculum), P₄ (20% basic substrate + 80% inoculum), P₅ (100% inoculum). These treatments were fermented with anaerob bacteria for 10 days in the fermentor 1000 mL mounted with the biogas collecting balloon. Variables observed in this study were the measurements of biogas volume, culture medium pH, and biogas burning temperature, and the testing of biogas flame colour. Based on the observation result, three of the six examined treatments which could able to produce biogas were P₂, P₃ and P₄ with the respective volume of 160 cm³, 143 cm³, and 118 cm³. While, the measured values of culture medium pH and biogas burning temperature were 6,87-7,10 and 152^oC with the flame of red colour. This study shows that the optimal mixture ratio of the basic substrate and the inoculum for producing biogas is 3:2, and the produced biogas is potential to be used as an alternative energy.